B-cell, myeloid, and mast cell development in Slug mutant mice.

Representative analysis of B cells and myeloid cells present in the spleen (A) and in the BM (B), respectively. Cells isolated from a wild-type (control), a heterozygous mutant (Slug+/−), a homozygous mutant (Slug −/−), a Steel mutant (Sl/Sl^d), and a W mutant (W/W^v) mouse were stained with monoclonal antibodies and were analyzed by flow cytometry. Percentage of cells is indicated. (C) Histologic examination was performed on ear sections from 4-week-old wild-type (+/+), homozygous mutant (Slug−/−), and W mutant (c-kit −/−) mice. All sections were stained with Giemsa.33 Arrowheads indicate the presence of mast cells in control and Slug−/− mice but not in W mutant mice. Original magnification × 40.