Fig. 7.
Epo (R103A) specifically inhibits erythroid precursors in human bone marrow culture.
Bone marrow mononuclear cells (1.2 × 106) were cultured in 1 mL methylcellulose culture medium containing Epo (23 ng/mL) or Epo (R103A) or both at 1 μg/mL (A) or 10 μg/mL (B). Cultures also contain IL-3, GM-CSF, and SCF. BFU-E and CFU-GM colonies were counted at day 14. “No addition” indicates that neither Epo nor Epo antagonist was added. Cultures were done in triplicate with SD indicated with the bars.
