Epo (R103A) does not induce apoptosis in 32D/EpoR cells.

32D/EpoR cells (300 000 cells/well) were cultured in triplicate in 24-well plates. Cultures contained 10% WEHI-conditioned media (as a source of IL-3), Epo (15.5 ng/mL), or Epo (R103A) (1 μg/mL) as indicated. Cells were harvested 24 hours later and apoptosis was measured using flow cytometry to detect exposure of annexin V binding sites.