Phenotypic characterization of ex vivo–expanded purified CD34⁺ cells.

CD34⁺ cells were cultured for 7 days in Iscoves modified Dulbecco medium containing FL, SCF, and TPO. CD34 and CD33 cell-surface expression was analyzed by cytofluorometry before (A) and after (B) ex vivo expansion. The percentage of each cell subpopulation is shown in the relevant area of each dot plot.