The effects of dexamethasone on agonist-induced CD63 translocation.
Representative images of human eosinophils single-stained with BODIPY-FL–conjugated secondary antibody (green) to detect immunoreactivity against CD63. (A,B) Immunofluorescence (CD63) staining of IFN-γ–stimulated eosinophils and DIC image of the same cell (time = 10 minutes). (C,D) Immunofluorescence staining of eosinophils preincubated with dexamethasone (10−6 M) prior to IFN-γ stimulation; the DIC image of the same cell. (E,F) Immunofluorescence staining of C5a/CB-stimulated eosinophils; DIC image of the same cell. (G,H) Immunofluorescence staining of eosinophils preincubated with dexamethasone (10−6 M) prior to C5a/CB stimulation; the DIC image of the same cell. (I,J) Immunofluorescence staining of IL-3/IL-5/GM-CSF–stimulated eosinophils; DIC image of the same cell. (K,L) Immunofluorescence staining of eosinophils preincubated with dexamethasone (10−6 M) prior to IL-3/IL-5/GM-CSF stimulation; the DIC image of the same cell. (M,P) Combined images of immunofluorescence staining of eosinophils with a combination of anti-CD63/BODIPY-FL and mouse IgG1/TRITC; the DIC image of the same cell (M,N). A combination of mouse IgG1/BODIPY-FL and anti-MBP/TRITC; the DIC image of the same cell (O,P). Original magnification is × 630 for all images.
