Fig. 2.
Fig. 2. Proliferative capacity and number of Lin−Sca1++kit+ and Lin−Sca1++kit− cells in B6 and DBA/2 mice. / (A) Number of cells and CFCs obtained after 5 days of liquid culture of 50 Lin−Sca1++kit+ cells from B6 and DBA/2 mice supported by KL, flt3L, and TPO. Results are given as mean ± SEM (n = 12 independent triplicate experiments; *significantly different from B6, paired t test). (B) Absolute number of Lin−Sca1++, Lin−Sca1++kit+, and Lin−Sca1++kit− cells in B6 and DBA/2 mice as determined by flow cytometric cell sorting (mean ± SEM, n = 8 independent experiments in which the bone marrow pooled from at least 2 mice was used; *significantly different from B6, paired t test). (C) Range of expression levels of Sca1 on Lin− bone marrow cells from B6 and DBA/2 mice.

Proliferative capacity and number of Lin−Sca1++kit+ and Lin−Sca1++kit− cells in B6 and DBA/2 mice.

(A) Number of cells and CFCs obtained after 5 days of liquid culture of 50 Lin−Sca1++kit+ cells from B6 and DBA/2 mice supported by KL, flt3L, and TPO. Results are given as mean ± SEM (n = 12 independent triplicate experiments; *significantly different from B6, paired t test). (B) Absolute number of Lin−Sca1++, Lin−Sca1++kit+, and Lin−Sca1++kit− cells in B6 and DBA/2 mice as determined by flow cytometric cell sorting (mean ± SEM, n = 8 independent experiments in which the bone marrow pooled from at least 2 mice was used; *significantly different from B6, paired t test). (C) Range of expression levels of Sca1 on Lin− bone marrow cells from B6 and DBA/2 mice.

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