Sort windows used to isolate Lin⁻Sca1⁺⁺, Lin⁻Sca1⁺⁺kit⁺, and Lin⁻Sca1⁺⁺kit⁻ cells.

Cells with low to medium forward scatter (FSC) and low side scatter (SSC) (R1), negative green (lineage) fluorescence, orange (Sca1) fluorescence higher than twice the fluorescence level of cells stained with control antibodies (not shown) (R2), and positive red (c-kit) fluorescence (LSK⁺, R3) or negative red fluorescence (LSK⁻, R4) were isolated. For the isolation of Lin⁻Sca1⁺⁺ cells, cells falling into R1 and R2 were isolated.