Effect of function-blocking mAbs against Mac-1 (CD11b/CD18) on spreading, adhesion, and cytolysis by PMN with hu14.18/GM-CSF.

ADCC with hu14.18/GM-CSF was as described in Figure 3 except that function-blocking mAbs were added just before the addition of hu14.18/GM-CSF. ADCC was performed in the presence of anti-CD18 7E4mAb (10 μg/mL) for (A) 30 minutes and (B) 120 minutes or anti-CD11b 2LPM19C mAb (10 μg/mL) for (C) 30 minutes and (D) 120 minutes. Electron micrographs were taken with an original magnification of × 5400.