Surface expression of chimeras of GPIX and GPIbβ.

HEK 293T cells transfected with wildtype GPIbβ (panel A) or the chimeras GPIX22ñ β15, (panel B) GPIX36/β32 (panel C), and GPIX53/49 (panel D) were reacted with MoAb 257.4. There is a significant increase in fluorescence in cells transfected with chimeras GPIX22ñ β15 and GPIX36/β32 and GPIX53/49. Thus, chimeras of GPIX and GPIbβ are surface expressed.