MoAb 257.4 recognition of an epitope in the N terminus 80 residues of GPIbβ.

The recombinant proteins GPIbβ and GPIbβ-trunc9 were expressed in HEK293T cells. The cells were lysed, analyzed by SDS-PAGE, and immunobloted with MBC 257.4. Lane 1 shows platelet lysate; lane 2, recombinant wildtype GPIbβ; lane 3, recombinant GPIbβ-trunc.9 The epitope for MBC 257.4 is within the N terminus 80 residues of GPIbβ.