Effect of PD169316 on MM.1S-cell apoptosis induced by NF-κB inhibition.

The p38 inhibitor PD169316 sensitizes MM.1S cells to apoptosis induced by NF-κB inhibition. Cell death was assayed by MTT in MM.1S cells treated with a nontoxic concentration of the p38 inhibitor PD169316 (10 μM) and/or the NF-κB inhibitor SN50 (10 μM) for 18 hours. PD169316 did not induce MM cell apoptosis, but did enhance the apoptotic effect of SN50, suggesting an interaction between the p38 and NF-κB pathways. Data shown (mean ± SD) are representative of 3 experiments.