Impaired myeloid differentiation of PLSCR1^−/−hematopoietic precursor cells.

Newborn (day 1) liver cells (A), adult spleen cells (B), and adult bone marrow cells (C), were cultured in MethoCult 3234 supplemented with 20 ng/mL SCF and 60 ng/mL G-CSF for either 8 (liver), 10 (spleen), or 7 (bone marrow) days, respectively. The cells were washed and transferred to glass slides by cytospin and stained with Wright-Giemsa solution. Slides were examined at × 1000 magnification.