Fig. 5.
Analysis of hematopoietic cell colony formation of cells from fetal liver, adult spleen, and adult bone marrow.
A total of 105 fetal liver cells (A), 2 × 105 adult spleen cells (B), or 5 × 104 adult bone marrow cells (C) were cultured in 1% methylcellulose supplemented with either a single cytokine or multiple cytokines as detailed in “Materials and methods.” Colonies were scored as clusters containing more than 50 cells at 6 to 9 days. Data are from 2 experiments, each performed with cells derived from 5 WT (■) and 5 PLSCR1−/− (□). *P < .05 and **P < .01 compared with WT.
