Absence of PLSCR1 in tissues from PLSCR1^−/− mice.

(A) Successful targeting of the PLSCR1 gene as confirmed by Southern blot of tail genomic DNA. (B) Northern blot of kidney mRNA. (C) Western blot shows absence of PLSCR1 antigen in bone marrow cells of homozygous PLSCR1^−/− mice. Blotting was performed as detailed in “Materials and methods.”