Influence of SDF-1α, GM-CSF, TPO, and SLF on survival of MO7e colony-forming cells (CFCs) after growth factor withdrawal.
MO7e cells were plated at 103 cells/mL in agar culture medium at time 0 with control medium or the amounts of SDF-1α, GM-CSF, TPO, or SLF, or SDF-1α plus either GM-CSF, TPO, or SLF shown. The combination of GM-CSF (10 ng/mL) plus SLF (50 ng/mL), a maximal concentration of factors to stimulate MO7e colony formation, was added to these plates at either time 24 or 48 hours. Results are shown as colonies ± SD (with the percent survival compared to time 0 culture plated with control medium shown in parentheses). Statistical differences between groups are noted by the same letters as for the results in Figure 7. The addition of either SDF-1, GM-CSF, TPO, SLF, or SDF-1 plus these cytokines along with the maximally stimulating combination of GM-CSF plus SLF at time 0 had no significant effect (P > .05) compared to control medium added with the maximally stimulating combination of growth factors at time 0 (data not shown).
