No effect of SDF-1α or other cytokines on translocation of NF-κB.

Factor-starved MO7e cells were stimulated with SDF-1α alone, other cytokines alone, or combinations of cytokines for 15 minutes, and the cellular localization of NF-κB was determined by confocal microscopy after staining with a mAb against human NF-κB p65. Nuclei were stained by Hoechst no. 33258. Cells treated with TNF-α (15 minutes) are shown as a positive control for NF-κB nuclear translocation. Magnification, × 100.