![Fig. 4. Kinetics of the effect of U46 619 and free AA on cytosolic Ca++ of erythrocytes. / RBCs loaded with fura-2 am (0.1% hematocrit in HEPES buffer) were placed in a quartz cuvette with stirring (37°C, 300 rpm) for fluorescence measurements (“Materials and methods”). Fluorescence was determined with excitation wavelengths 340 and 380 nm and 510 nm emission wavelength in a spectrofluorimeter. After determination of baseline [Ca++]i, U46 619 (1 μM), free AA (20 μM), ionomycin (1 μM), or solvent (controls) was added. [Ca++]i of erythrocytes was quantified at 1-second intervals for 10 minutes. The figure shows time-dependent [Ca++]i changes. Data are representative of 11 separate experiments in different donors. PGI2 (0.5-1 μM) or 12-HETE (0.1-1 μM) did not increase [Ca++]i over that of solvent control (not shown).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/11/10.1182_blood.v99.11.3978/6/h81122629004.jpeg?Expires=1724259053&Signature=A-vtBXvidP1~3TdT-vNlkmLnOiSqZKyH5iWqGJHUStMNUYU7guZSDticIPzoYDKBikXbgkJ8MPoIHgHX-XRhbWsvQkthMu~yr-aDWVlajNOoLoD~5WgCldVruLpQWavhei09HPIPEx5yG6KLv7nZWJnqVCGkBBXP4H2djSQWBbuKd4ouYG3K~kbihYAxJtz0efyDUtjVPbfQ9uwKBLcwkh1YdUtbsY2poS-qOnu~7pLIfNQGR3MeKvdD0ma4m7D53WafUS9lcNEPUgjgPeOOlq99RtdA6e4ZEdbYDIDSOjWB-VMbzRSiF8JqxeN~3~uB-ubr6b95rePJ5glVcXVS8Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Kinetics of the effect of U46 619 and free AA on cytosolic Ca++ of erythrocytes.
RBCs loaded with fura-2 am (0.1% hematocrit in HEPES buffer) were placed in a quartz cuvette with stirring (37°C, 300 rpm) for fluorescence measurements (“Materials and methods”). Fluorescence was determined with excitation wavelengths 340 and 380 nm and 510 nm emission wavelength in a spectrofluorimeter. After determination of baseline [Ca++]i, U46 619 (1 μM), free AA (20 μM), ionomycin (1 μM), or solvent (controls) was added. [Ca++]i of erythrocytes was quantified at 1-second intervals for 10 minutes. The figure shows time-dependent [Ca++]i changes. Data are representative of 11 separate experiments in different donors. PGI2 (0.5-1 μM) or 12-HETE (0.1-1 μM) did not increase [Ca++]i over that of solvent control (not shown).
