Identification of the proximal promoter region of thep40^phox gene.

HL-60 cells in log phase of growth were transfected with the indicated constructs and assayed for luciferase activity after 48 hours. Allp40^phox constructs extended from the indicated position in the 5′-flanking sequence of the gene to nucleotide +104 of the 5′-UTR relative to the reported transcriptional start site.36 Luciferase activity is reported as the ratio of the test construct to the promoterless vector pGL3-Basic. Values were corrected for transfection efficiency by cotransfection with the renilla expression plasmid and were normalized to equal molar content of DNA. Data shown are means (± SE) of 5 independent experiments.