Overview of several CpG islands from the critical region that was tested for DNA methylation.

CpG doublets localized in the promotor regions of the BCL2, KPNA3, RFP2, BCMS, and BCMSUN genes are depicted as vertical lines in the genomic sequence (base pair [bp] in numbers). In addition, Msp1 restriction sites (5′-CCGG-3′) of the genomic region of the 5′-ends of the BCMS and BCMSUN genes are shown at a lower magnification. Arrows indicate localization and direction of genes; exons are shown as black or gray boxes. Primer pairs that were used in quantification of methylation with real-time PCR are shown as short black lines A to K. Dotted black lines show regions that were bisulfite sequenced.