Methylation suppresses ITGAL promoter function.

The regions from −1818 to −362 and −362 to +79 were excised, methylated with SssI in vitro, ligated back into a luciferase reporter construct, and transfected into Jurkat cells, using cotransfection with β-galactosidase as a control. Controls consisted of similar preparations but without the addition of SssI. The results are presented as the ratio of luciferase/β-galactosidase expression in arbitrary units standardized to the mock-methylated controls and represent the mean ± SEM of 5 experiments for the region from −1818 to −362 and 4 experiments for the region from −362 to +79.