American Society of Hematology

Fig. 3.

$Fig. 3. Chromatin structure is condensed around theITGAL locus in fibroblasts but not T cells. / Freshly isolated T cells and 2 × 107 fibroblasts were homogenized in phosphate-buffered saline and then treated with 0, 40, 80, or 160 U/mL DNase1. DNA was then isolated, digested withSacI, fractionated by agarose gel electrophoresis, transferred to nylon filters, hybridized with a 32P-labeled complementary DNA probe amplified from bp 1060 to 1264 of theITGAL gene, and developed using a PhosphorImager. Fragment size is shown in the right column, and the figure at the bottom shows the 3.2-kb SacI fragment, the relative size and location of the probe, and the approximate location of the 1.4 kb fragment. The broken arrow represents the transcription start site.$

Chromatin structure is condensed around theITGAL locus in fibroblasts but not T cells.

Freshly isolated T cells and 2 × 10⁷ fibroblasts were homogenized in phosphate-buffered saline and then treated with 0, 40, 80, or 160 U/mL DNase1. DNA was then isolated, digested withSacI, fractionated by agarose gel electrophoresis, transferred to nylon filters, hybridized with a ³²P-labeled complementary DNA probe amplified from bp 1060 to 1264 of theITGAL gene, and developed using a PhosphorImager. Fragment size is shown in the right column, and the figure at the bottom shows the 3.2-kb SacI fragment, the relative size and location of the probe, and the approximate location of the 1.4 kb fragment. The broken arrow represents the transcription start site.

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