Botrocetin-induced VWF binding to control and the Del mutant cells.

(A) Control cells and (B) wild-type and Del cells were incubated with increasing concentrations of VWF (0-2 μg/mL) in the presence of 2 μg/mL botrocetin. Bound VWF was detected using an FITC-conjugated antihuman VWF antibody, followed by flow cytometric analysis. (A) The data are presented as mean fluorescence plotted against increasing VWF concentration. (B) The data are expressed as a ratio of the mean fluorescence of VWF binding to that of AK3 binding. The mean ratio ± SEM from 3 experiments is shown.