RNase protection assay in human PBLs.

An RNase protection assay was performed as described. Antisense mRNA for probe (288 bp), part of caspase-8L mRNA, caspase-8a mRNA, and protected bands (178 bp and 158 bp) are schematically shown on the left. The probe includes a portion of 136 bp insertion and a portion of exon 9. Lane 1, size marker; lane 2, an undigested probe; lane 3, a probe digested after hybridization with 20 μg RNA of PBLs from a healthy human individual; lane 4, probe digested after hybridization with 20 μg tRNA (negative control). The base pair length demonstrated on the right was derived from sequencing reactions.