Fig. 9.
Binding of GST-αMI to wild-type Cyr61 and Cyr61-DM.
Microtiter wells were coated with 30 μg/mL BSA, Cyr61-WT, or Cyr61-DM, and blocked with 0.15% PVA. GST-αMI (1 μM), preincubated with the indicated concentrations of heparin, was added to the wells and allowed to bind for 2 hours at 22°C. Bound GST-αMI was detected by an ELISA using an anti-GST polyclonal antibody as described in the legend of Figure 8. Data shown are means of triplicate determinations and error bars represent SEs. Figure is representative of 2 experiments.
