Specificity of immunohistochemical staining of Cyr61 in mouse atherosclerotic lesions.

Frozen serial sections, taken from the hearts of ApoE^−/−mice, were incubated with rabbit IgG (A) or anti-Cyr61^367–381 (B,C). To demonstrate staining specificity in panel C, anti-Cyr61^367–381 was preabsorbed with 5 μg/mL recombinant Cyr61 for 16 hours at 4°C prior to its application to the sections. Immunohistochemical staining was performed as described in the legend of Figure 1.