Discrepancy between donor T-cell expansion and antihost lymphohematopoietic alloreactivity in recipients of GKO BALB/c CD8 T cells.

WBCs were prepared from nonleukemic recipients of 5 × 10⁶ TCD B6 BMCs plus 5 × 10⁶ TCD GKO BALB/c BMCs and 1 × 10⁶ purified CD8⁺splenocytes from WT (▪; n = 10) or GKO (■; n = 10) BALB/c mice at indicated times. Levels of donor CD8 T (5F1⁻CD8⁺) cells (A) and total host type (5F1⁺, ie, H-2K^b+) cells (B) in the WBCs, and percentages of host B cells (5F1⁺CD19⁺) in the B-cell (CD19⁺) population (C) were determined by FACS. Data are presented as group means (±SD). These mice are the same nonleukemic WT BALB and GKO BALB recipients shown in Figure 4A. *P < .05, **P < .01, ***P < .0001.