Allogeneic CD8 T cells induce more severe systemic GVHD but less marked GVL effects if they are incapable of IFN-γ production.
Survival is shown for nonleukemic and leukemic B6 recipients of 5 × 106 TCD B6 BMCs (syngeneic), or 5 × 106 TCD B6 BMCs plus 5 × 106 TCD GKO BALB/c BMCs and 1 × 106 (A) or 2.5 × 106(B) WT BALB/c CD8+ splenocytes (WT BALB), or 5 × 106 TCD B6 BMCs plus 5 × 106 TCD GKO BALB/c BMCs and 1 × 106 (A) or 2.5 × 106(B) GKO BALB/c CD8+ splenocytes (GKO BALB). Leukemic recipients were injected with 500 EL4 cells along with the BMT inoculum. Survival data from 2 independent experiments (panels A and B) are shown and the numbers of mice in each group of the 2 experiments are indicated inside parentheses (a and b are mouse numbers of the indicated group in experiment A and experiment B, respectively).
