MIP-1β binding is inhibited by BCD treatment.

CEM-R5 cells were treated with BCD (A) and stained with biotinylated MIP-1β and detected with avidin-fluorescein conjugate as described in “Materials and methods.” Relative fluorescein (FITC) fluorescence is expressed on the x-axis. The results for biotinylated-negative control are graphed as a filled peak, MIP-1β binding to untreated cells is graphed as a solid line, and MIP-1β binding to BCD-treated cells are graphed as a dashed line. M1 indicates the range of cells with FITC fluorescence more than 98% of the negative control. One representative experiment of 4 is presented. (B) CEM-R5 cells were treated with the indicated concentrations of BCD for 1 hour. Cholesterol quantitation of cells was performed exactly according to Molecular Probes' Amplex Red Cholesterol Assay Kit. Results are expressed as cholesterol concentration per input of cells.