American Society of Hematology

Fig. 5.

$Fig. 5. Association of the cytoplasmic domain of PSGL-1 with the actin cytoskeleton is disrupted in PSGL-1Δcyto transfectants. / The detergent fractionation assay was performed as described in “Materials and methods.” High levels of KPL1 staining remained in the detergent-insoluble pellet associated with PSGL-1 transfectants (left panels), but this staining was greatly reduced in PSGL-1Δcyto cells (right panels). Top, untreated cells; bottom, detergent-treated cells.$

Association of the cytoplasmic domain of PSGL-1 with the actin cytoskeleton is disrupted in PSGL-1Δcyto transfectants.

The detergent fractionation assay was performed as described in “Materials and methods.” High levels of KPL1 staining remained in the detergent-insoluble pellet associated with PSGL-1 transfectants (left panels), but this staining was greatly reduced in PSGL-1Δcyto cells (right panels). Top, untreated cells; bottom, detergent-treated cells.

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