Effect of AT III on TF gene expression in monocytes and ECs.

Cells were pretreated for 2 hours with the indicated concentrations of AT III, followed by stimulation with either LPS (monocytes; 10 μg/mL) (A) or TNF-α (ECs; 40 ng/mL) (B). Cells were harvested after 4 hours of stimulation, and RT-PCR was performed with primers specific for TF or β-actin gene. A representative ethidium bromide visualization of TF and β-actin mRNA is given in the upper panel. Relative values of TF mRNA (normalized to β-actin) are given in the lower panel. The values represent means ± SEM of 4 independent experiments. *P < .01 versus stimulation in the absence of AT III.