Effect of AT III on the agonist-induced phosphorylation and degradation of IκBα in monocytes and ECs.

Cells were pretreated for 2 hours with the indicated concentrations of AT III, followed by stimulation with either LPS (monocytes; 10 μg/mL) (A) or TNF-α (ECs; 40 ng/mL) (B) in the presence and absence of AT III. Cytoplasmic proteins were prepared, and immunoblotting was performed for the phosphorylated form of IκBα (P-IκBα) and for IκBα. In each case, data are representative of 3 independent experiments.