Effect of Trp⁴⁹-modified AT III, latent AT III, β–AT III, and α–AT III on NF-κB activation in monocytes and ECs.

Cells were pretreated for 2 hours with the indicated AT compounds, followed by a 1-hour stimulation with LPS (monocytes; 10 μg/mL) (A) or TNF-α (ECs; 40 ng/mL) (B) in the presence and absence of the compounds. Nuclear proteins from monocytes and ECs were analyzed by EMSA for binding to an oligonucleotide containing the NF-κB consensus sequence.