Fig. 2.
Effect of AT III on DNA-binding activity of NF-κB and AP-1 in monocytes and ECs.
Cells were pretreated for 2 hours with different concentrations of AT III, followed by a 1-hour stimulation with either LPS (monocytes; 10 μg/mL) (A) or TNF-α (ECs; 40 ng/mL) (B) in the presence and absence of AT III. Nuclear extracts were analyzed by EMSA with33P-labeled DNA probes detecting κB and AP-1 binding activities, respectively. Representative photographs of EMSA gels are shown. Complexes tested for specific binding are indicated by arrows.
