Influence of solubilizing detergents on c-kit/TM4SF association.

(A) Cells were solubilized with lysis buffer containing 1% indicated detergents. Cell lysates were immunoprecipitated with anti-CD63 or anti-CD81 mAb. Immunoprecipitated proteins were separated by SDS-PAGE and immunoblotted with anti–c-kit polyclonal antibody. (B) Cells were solubilized with lysis buffer containing 1% NP40 plus 0.1% SDS plus 0.5% sodium deoxycholate with or without pretreatment with 0.5 mM DSP and submitted to immunoprecipitation with indicated antibodies. After being separated by SDS-PAGE, proteins were immunoblotted with anti–c-kit polyclonal antibody. These are both the representative results from 3 separate experiments.