Association of c-kit with TM4SF molecules in MO7e cells.

Cells were solubilized with lysis buffer containing 1% CHAPS. (A) Cell lysates were immunoprecipitated with normal rabbit IgG or anti–c-kit polyclonal antibody. After being separated by SDS-PAGE under nonreducing conditions, proteins were immunoblotted with anti-CD9, anti-CD63, or anti-CD81 mAb. (B) The cell lysates were immunoprecipitated with indicated antibodies. Anti-CD50 (ICAM-3) mAb was used as negative control. After being separated by SDS-PAGE, proteins were immunoblotted with anti–c-kit polyclonal antibody. These are the representative results from 3 separate experiments.