Inhibition of the transfected cell adhesion by the Moab AP-2 or the peptide RGDS.

Transfected cells expressing the Duv^a+ or the Duv^a− forms of the GP IIb-IIIa complex were incubated in microtiter plates coated with 20 μg/mL fibrinogen. The incubation was performed in PBS-albumin (control) and in the presence of 20 μg/mL of either the Moab AP-2 or a nonrelevant polyclonal mouse IgG (A) or in the presence of 5 mM of the RGDS or RGES peptides (B). Quantification of bound cells was performed as described in the legend to Figure 7. The adhesion of CHO cells expressing the Duv^a+ GP IIIa form of the complex is inhibited by the Moab AP-2 or the peptide RGDS. Results represent the mean ± SD of 3 experiments.