Fig. 5.
![Fig. 5. DNA analysis of transduced adenoviral vector DNA in genomic liver DNA. / C57Bl/6 mice were injected with 2 × 109 transducing particles of either AdFTC/hFIX or fgAdhFIX. At both 5 days and 1 year after injection, genomic liver DNA was isolated and digested with HindIII, and a Southern blot was performed (n = 2 per group). Blots were hybridized with an α-32]dCTP–labeled cDNA hFIX probe, and the copy was determined by comparing the signal intensity of the resulting band with a standard curve that was obtained by digesting known amounts of the plasmid pBSh7hF9mgbpA containing the identical expression cassette withHindIII.19](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/11/10.1182_blood.v99.11.3923/6/h81122627005.jpeg?Expires=1724250443&Signature=ndQVvMWBR4W9DpkYJOUNCJIN6xQWe6T1WY~TUD9z91QItLxixODwrtU7lbErBzl25QAM99a8qqvl-V6blJGx6D0WTSix0vJUbpcvV5a8wJJufN1C5yFKTLULP3S3OrehhyZmSZoOHaCd~PmYC0rBlhGS~QNtBHVGkyUxgEmp6MFzfW14VOzsORDoDBRA3O-cwig8rljEtp6za9Ng7n1v1SfNkfpotnVd--U-8oCsAQUa4nvqU3OS1EZDzsk9OeQUYDlqn5Zoz0mP9~mgdsyCYVvdFUUJ~T2uf0pvu8yz5Oz94HIOPvIE6JI9l3lcwSDmJiDIzIsFjYixsFTDIs9roQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
DNA analysis of transduced adenoviral vector DNA in genomic liver DNA.
C57Bl/6 mice were injected with 2 × 109 transducing particles of either AdFTC/hFIX or fgAdhFIX. At both 5 days and 1 year after injection, genomic liver DNA was isolated and digested with HindIII, and a Southern blot was performed (n = 2 per group). Blots were hybridized with an α-32]dCTP–labeled cDNA hFIX probe, and the copy was determined by comparing the signal intensity of the resulting band with a standard curve that was obtained by digesting known amounts of the plasmid pBSh7hF9mgbpA containing the identical expression cassette withHindIII.19
