Long-term comparison of the hFIX expression levels of the HD adenovirus AdFTC/hFIX and the first-generation adenovirus fgAdhFIX in vivo in C57Bl/6 mice and C57Bl/6 hemophilia B mice.

(A) First, 2 × 10⁹ transducing particles of AdFTC/hFIX (●) or fgAdhFIX (○) (n = 3 per group) were injected into mice. (B) C57Bl/6 hemophilia mice were injected with 1 × 10⁹transducing units AdFTC/hFIX (▪) or fgAdhFIX (■); 5 × 10⁸ transducing units AdFTC/hFIX (●) or fgAdhFIX (○); or 1 × 10⁸ transducing units AdFTC/hFIX (▴) or fgAdhFIX (▵) (n = 3 per group). Serum hFIX levels were periodically determined.