Fig. 1.
The DNA enzyme efficiently cleaved its target mRNA–Raf-1.
The DNA enzyme was incubated with internally labeled 770 nt mRNA–Raf-1. Samples were taken at indicated time points and then analyzed with 10% polyacrylamide gel. The active DNA enzyme degraded 32P internally labeled mRNA–Raf-1 in vitro. No enzyme was added in the left and right lanes. The upper bands represent the Raf-1 mRNA, while the lower bands represent the 5′-cleavage product.
