Effect of EMPs on TF protein level in THP-1 cells.

THP-1 cells were incubated with control medium (CM), EMP, or EMP-free supernatant (S) for 15 minutes to 4 hours, and TF antigen level in cell lysates was determined using ELISA. At 4 hours, LPS stimulation was used as positive control. The quantity of antigen was expressed in pg/mL cell lysate. Values are mean ± SD (n = 4). *P < .05; **P < .01.