Fig. 7.
Antisense knockdown of bcl-2 leads to drastic human blood B-cell apoptosis.
Blood B cells were isolated from buffy coats by negative magnetic selection, cultured, and treated for 24 hours with DMRIE-C alone (24 μL per 2 × 106 cells) or DMRIE-C combined withbcl-2 AS or SC ODNs (8 μg per 2 × 106cells). (A) Whole-cell extracts were analyzed by immunoblotting for Bcl-2 and Bax. (B) Alternatively, apoptosis assays using dual-color annexin-V–FITC/PI staining and flow cytometry analyses were performed on treated B cells. The percentage of single- or double-positive cells in the individual quadrants is shown. These results were representative of at least 3 comparable experiments.
