Complete NF-κB inhibition results in Bcl-2 down-regulation in resting human blood B cells.

(A) Blood B cells were isolated from buffy coats by negative magnetic selection and cultured for 6 hours and 24 hours in the presence or absence of 1.5 μM mGTX, 1.5 μM GTX, or 48 μM PGA1. Whole-cell extracts were analyzed by immunoblotting for Bax and Bcl-2 expression. (B) RNA prepared from blood B cells cultured for 6 hours and 18 hours in the presence or absence of 1.5 μM mGTX or 1.5 μM GTX was analyzed by RT-PCR for expression of bcl-2. To control for quantification, GAPDH was amplified. These results represent at least 3 comparable experiments.