Ultrastructural immunonanogold localization of human cellubrevin in unstimulated human platelets.

Most silver-enhanced gold particles are attached to α-granule membranes (A, arrowheads; B). Label is also present on OCS (A, open arrows) and plasma membrane (A, solid arrow). (C) Absorption control of the specific primary antibody with cellubrevin is negative. (D) Irrelevant rabbit IgG was substituted for the specific primary antibody, and no labeling is seen. Original magnifications: A, × 42 000; B, × 86 000; C, × 56 000; D, × 48 000.