MLL-AF10 immortalizes murine myeloid progenitors.

(A) Morphology of colonies formed in methylcellulose byMLL-AF10–transduced cells (May-Grünwald/Giemsa stain, × 10). (B) May-Grünwald/Giemsa–stained cytospin preparation shows cells with a range of primitive myeloid morphology (× 50). (C) RT-PCR analysis shows the presence ofMLL-AF10 transcripts in immortalized cells. (D-G) Fluorescence-activated cell sorter analysis ofMLL-AF10–transduced cells was performed after third-round cultures. Percent of cells expressing the indicated surface antigens is indicated.