Fig. 1.
Fig. 1. Retroviral transduction of MLL-AF10enhances the in vitro replating potential of primary murine BM cells. / (A) Diagrammatic representations of the MLL and AF10 proteins are shown with known structural motifs indicated. Translocation t(10;11)(p12;q23) joins the amino-terminal portion of MLL to the carboxy-terminal portion of AF10 containing its LZ motif. (B) Schematic representation of the retroviral transduction protocol. (C) Colonies generated per 104 transduced BM cells were determined in first-, second-, and third-round cultures for MLL5′,MLL-AF10, and FLAG-AF10 constructs. First-round results indicate G418R colonies. Bars represent the mean ± SD for 3 experiments.

Retroviral transduction of MLL-AF10enhances the in vitro replating potential of primary murine BM cells.

(A) Diagrammatic representations of the MLL and AF10 proteins are shown with known structural motifs indicated. Translocation t(10;11)(p12;q23) joins the amino-terminal portion of MLL to the carboxy-terminal portion of AF10 containing its LZ motif. (B) Schematic representation of the retroviral transduction protocol. (C) Colonies generated per 104 transduced BM cells were determined in first-, second-, and third-round cultures for MLL5′,MLL-AF10, and FLAG-AF10 constructs. First-round results indicate G418R colonies. Bars represent the mean ± SD for 3 experiments.

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