Flow cytometry of FITC-FXII expression on HUVECs.

Flow cytometry was performed with suspensions of washed, unfixed, and nonpermeabilized HUVECs (5 × 10⁶/mL). HUVECs were incubated for 1 hour at 37°C in HCB containing 10 μM ZnCl₂ in the presence (shaded curve) or the absence (dotted curve) of 70 nM FITC-FXII or in the presence of 70 nM FITC-FXII (solid curves) and mouse anti-uPAR (4 μg/mL), goat anti-GPV20 (300 μg/mL), mouse anti-gC1qR (4 μg/mL), goat IgG (300 μg/mL), or mouse IgG (4 μg/mL). The binding of these antibodies to HUVECs was detected by a shift in the solid curve to the left in comparison to FITC-FXII alone (shaded curve). The figure is a representative presentation of 3 independent experiments.