Visualization of Nanogold-FXII on EA.hy926 endothelial cells.

Cell suspensions were incubated for 60 minutes at 37°C with 210 nM Nanogold-FXII in HCB, pH 7.4, containing 10 μM Zn²⁺, and then gold particles were enhanced with silver. Panel A shows the silver-enhanced FXII (arrows) at the light microscopic level. Panel B represents a low-power electron microscope view of an endothelial cell with clusters of silver-enhanced FXII on the cell surface (arrows). Panel C shows a high magnification of the irregular silver-enhanced Nanogold-FXII particles (arrows).