Laser scanning confocal microscopy of FITC-FXII binding to HUVECs.

Nonpermeabilized, paraformaldehyde (2%)–fixed HUVECs were grown on microscope slides. HUVECs were incubated with 300 nM FITC-FXII in HCB in the presence or absence of 10 μM ZnCl₂ or 100-fold molar excess of unlabeled FXII for 1 hour at 37°C. Detection of FITC-FXII on the HUVECs is described in “Materials and methods.” The panels are photomicrographs of the laser scanning confocal microscopy. The figure is a representative presentation of 3 independent experiments.