Fig. 4.
Fig. 4. The impaired capability of T-helper cells to be induced to express CD69 upon nonspecific stimulation affects both the naive (CD4+CD45RA+) and the memory (CD4+CD45RA−) population after T-cell depletion. / PBMCs of control samples (upper panels) and of patient samples (lower panels) were stimulated with immobilized anti-CD3. At 24 hours of culture, T-helper cells were identified by anti-CD4 and gated and assessed for expression of CD45RA. The CD45RA+ and the CD45RA− populations were gated and separately analyzed for the expression of CD69. The density of expression of CD69 was estimated by the mpc in histogram plots. Patient samples were obtained from a 5-year-old boy with stage IV Ewing sarcoma (patient 15) 3 months after high-dose chemotherapy with autologous stem cell rescue. CD4+CD45RA+ cells, 126/μL.

The impaired capability of T-helper cells to be induced to express CD69 upon nonspecific stimulation affects both the naive (CD4+CD45RA+) and the memory (CD4+CD45RA−) population after T-cell depletion.

PBMCs of control samples (upper panels) and of patient samples (lower panels) were stimulated with immobilized anti-CD3. At 24 hours of culture, T-helper cells were identified by anti-CD4 and gated and assessed for expression of CD45RA. The CD45RA+ and the CD45RA− populations were gated and separately analyzed for the expression of CD69. The density of expression of CD69 was estimated by the mpc in histogram plots. Patient samples were obtained from a 5-year-old boy with stage IV Ewing sarcoma (patient 15) 3 months after high-dose chemotherapy with autologous stem cell rescue. CD4+CD45RA+ cells, 126/μL.

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