Immune reconstitution in NOD/SCID-Tg mice receiving T- and B-cell–depleted B10.D2/o-Tg mouse BMCs.
NOD/SCID-Tg mice were treated with 3 Gy WBI and some of them received subsequent infusion of 1-1.5 × 106 T- and B- cell–depleted B10.D2/o-Tg mouse BMCs at week 5, 7, or 11 after WBI. (A) Rejection of skin grafts from allogeneic mouse and xenogeneic porcine donors by B10.D2/o-Tg mouse BMC-reconstituted NOD/SCID-Tg mice. B10.RIII mouse and porcine skins were grafted simultaneously in NOD/SCID-Tg mice 2 weeks after administration of T- and B-cell–depleted B10.D2/o-Tg mouse BMCs. Survival is shown for B10.RIII (dashed line) and pig (solid line) skin grafts in irradiated NOD/SCID-Tg mice receiving (▴; n = 8) or not receiving (▪; n = 8) a subsequent infusion of B10.D2/o-Tg mouse BMCs. Both B10.RIII and porcine skin grafts were accepted in NOD/SCID-Tg recipients of WBI only for over 100 days or until death (*), but were rejected by B10.D2/o-Tg mouse BMC-reconstituted NOD/SCID-Tg mice by 42 days. (B). Development of mouse CD4+ cells, CD8+ cells, and surface IgM+ cells in NOD/SCID-Tg mice reconstituted with T- and B-cell–depleted B10.D2/o-Tg mouse BMCs. WBCs and splenocytes were prepared from B10.D2/o-Tg mouse BMC-reconstituted mice (●) and control animals that received WBI only (○) during the period of 10 to 15 weeks after B10.D2/o-Tg mouse BMC infusion, and percentages of mouse CD4+, CD8+ and surface IgM+ cells were measured by 2-color FACS analysis. Each symbol represents an individual animal. Data are pooled from 4 independent experiments.
